Another two β-amylases (BA3 and BA4), which are encoded in the T.
The secretion was inhibited by brefeldin A but not by FLI-06. We demonstrated that two β-amylases (BA1 and BA2) are transported via the classical endoplasmic reticulum-to-Golgi pathways, and in the case of BA1, we showed that the protein is glycosylated with multiple N-linked glycans of Hex 5HexNAc 2 structure. Therefore, we used β-amylases as a model to investigate the T.
Interestingly, current bioinformatic tools predicted the secretory signal in only 18% of the identified T. The other half of the soluble proteins include several novel potential virulence factors, such as DNaseII, pore-forming proteins, and β-amylases. These proteins mainly include putative adhesins and leishmaniolysin-like metallopeptidases. Approximately half of the secreted proteins were predicted to possess transmembrane helixes. These 89 bona fide secreted proteins were sorted into 13 functional categories. In total, we identified 2 072 extracellular proteins, 89 of which displayed significant quantitative increases over time at 37 ☌. vaginalis secretome, considering that secretion is a time- and temperature-dependent process, to define the cutoff for secreted proteins. In this study, we used high-resolution label-free quantitative MS to analyze the T. vaginalis secretome remains elusive, as do the mechanisms of protein secretion. However, a comprehensive profiling of the T. Several effector proteins are known to be secreted by Trichomonas vaginalis, a human parasite of the urogenital tract. The secretion of virulence factors by parasitic protists into the host environment plays a fundamental role in multifactorial host–parasite interactions.
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